![]() ![]() Efektiv Pty Ltd v Hodge VSC 636 - appeal from Associate Judge – application to stay order of VCAT pending application for leave to appeal. ![]() In the matter of Orico Australia Pty Ltd VSC 313 – corporations, derivative action.Draper v Building Practitioners Board and Anor VSC 866 – administrative law, adequacy of reasons for decision. ![]()
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![]() ![]() The purchaser, user or participant assumes all risk of injury from any use. Cooper Hunting disclaims any and all liability related to improper, illegal, unintended use or modification of weapons sold through our website or catalog. By purchasing any item from our site - the buyer warrants compliance with all local, state, and federal law, and that the buyer is legally able to purchase such items from our website or catalog. Cooper Hunting will not be held liable for the misuse of any product listed on this website. Also, any attempt in entrapment by any law enforcement agency will be met with swift legal action. Erases rust, tarnish and other surface blemishes on metal, and produces a fine, brushed satin finish. All equipment is sold subject to public law 90-351, title III, U.S.D., Section 2511, and any local, state or federal ordinances. It's the responsibility of the buyer, NOT THE SELLER, to ascertain, and obey, all applicable local, state, and federal laws in regard to the possession and use of any item on this site. Erases rust, tarnish and other surface blemishes on metal, and produces a fine, brushed satin finish. It's YOUR responsibility to know the law before you order. Super Products 0101 - Single Rust Eraser with Brushed Satin Finish. Suspected items are not to be used as weapons or to cause bodily harm or damage to personal property in any way. Many states allow you to own suspected items but not carry them. As the purchaser of these types of knives, it is your responsibility to ensure that. *Sales Disclaimer: If you are not sure about the legality of any item for sale on our website, you MUST contact your local District Attorney's Office and ask them if you can carry or own suspected items in your state. Know the laws in your area prior to purchase. All Rights Reserved. Cooper Hunting does not accept liability for incorrect spelling, printing errors (including prices), incorrect manufacturer's specifications or changes, or grammatical inaccuracies in any product included on the Website. Any import duties, taxes, or charges are not included in the price or shipping and are the buyer' s responsibility.Entire contents Copyright ©2023 Cooper Hunting. International Customers: Please note you are responsible for knowing the import laws, fees, and taxes on the item being imported. Most of our items ship via USPS or UPS within 1-3 days from date payment is received. Dance classes slc utah, Itch eraser review, Estudio delloni sunchales. But caution it will scratch the metal it must in order to remove. Gp referral to gym, Beebs livermore breakfast, Super campeones 2002 capitulo 32. I got for knife blades for when i notice some spots of small rust. Polishing, cleaning and sharpening products designed to keep your knife in the best possible condition. We use All payments are due within 5 days after the auction ends. Works nicely and removes rust with ease along with any other dirt or grime. Super - Rust Eraser - Super (tm) premium line of cutlery care products. Seller in no way accepts responsibility for, nor assumes any liability in the use of this item. By bidding on and/or purchasing this item you certify that you are at least 18 years of age and are in compliance with all federal, state, and local laws pertaining to your possession, and the shipping of this product to your location. ![]() We've been selling on ebay for over 12-years now & have been blessed with thousands of excellent customers.many who return time & time again! We appreciate each & every customer we have and hope to do business with you! Thank you & please send us any questions you may have concerning this auction. ![]() Super Knife Rust Eraser Cleaner Lot of 2 New With Manufacturers Warranty SR0101: A lot of 2- Super Knife Rust Eraser / Cleaner. If you want to be a bit of a Super Hero then you need this Super Eraser Rust and Tarnish Eraser (SR0101). ![]() ![]() ![]() Pork Tenderloin & Potatoes With Creamy Mushroom Sauce Made with your choice of beans, vegetables and a little bit of ground pork, this dish has deep flavours and is a nourishing staple you’ll be revisiting often. Now, we’re in Italy for this beautifully hearty stew from the Tuscan region. We’ve made our way through Mexico, Greece, India, Germany, and all throughout Asia. It’s recommended that you give them a quick broil in the oven or on the grill before serving to seal in that flavor, but you won’t have to wait hours and hours for a tender, juicy, fully cooked inside now. ![]() You can do it the old-fashioned way, but once you start cooking the meat in your IP, you won’t go back. This delicious rack of smoky Instant Pot barbecue ribs is low-carb and keto-friendly. These juicy chops are smothered in a mushroom gravy that’ll have you drooling before you even take a bite! Get the recipe on the blog here. Getting the cooking time just right can be tricky when avoiding dry chops, but this recipe leaves you with little guesswork for brilliant results every time. Who doesn’t love a good pork chop? This is an underrated protein choice in my opinion. Top it off with fresh lime and cilantro for the best results! Get the recipe on our blog here.Ģ.Pork Chops With Creamy Mushroom Sauce (Keto, GF) It’s perfect for constructing your own healthy burrito bowls with rice and beans, wraps, or tacos. If you like Chipotle, you’ll love this pork dish. Barbacoa Inspired Instant Pot Pulled Porkįeeling like Mexican food? Look no further than this shreddable pulled pork, Barbacoa-style. Here are our favorite Instant Pot pork recipes for you to try! 1. We’ll be covering so many different types of cuisines that you’re bound to find your flavor and feel inspired. Whether you’re a fan of pulled pork, pork chops, or finger-lickin’ good ribs, we have the right recipe for you to try in your Instant Pot soon. There are many budget-friendly cuts to work with, it takes on any flavor profile and sauce brilliantly, and the end result is almost always hearty and satisfying. ![]() It is a truly underrated ingredient when we consider its versatility, affordability, and incredible taste. Let’s talk about pork! When it comes to the main animal proteins, I feel like pork gets left out the most often. ![]() ![]() Apple Pay is perfect for quickly grabbing a coffee or sandwich on the run, since you can set it to charge your corporate card-great for avoiding the “it’s in here somewhere” search through an over-packed laptop bag. ![]() You can even use Passbook on Apple Watch to scan your boarding pass. Concur’s TripIt and Apple’s Passbook take it a step farther, adding hotel information to flight itinerary glances.Use airline apps, like British Airways and Fly Delta, to see your itinerary and gate information at a glance.A few of my favorite Apple Watch travel tips: This is where the world of apps really comes into play. Take control of the business travel chaos. I can take a peek at incoming calls, see if an email needs immediate attention or respond to my daughter’s text with a happy-face emoticon-all without staring rudely at a screen. I love Apple Watch notifications for just this purpose. That said, work doesn’t stop because you’ve blocked out time on your calendar. Maybe it’s because of my Southern-mother upbringing, but if it’s important enough to drop everything and spend 30 minutes face-to-face, it’s important enough to be 100% engaged. Although the Watch audio works fine for personal communication, I still take professional calls on my iPhone to avoid any mishaps. The meeting shows up on the watch face, you click it and you’re dialed in. Calendaring apps like MobileDay or Agenday rid our lives of complicated passcodes and dial-in numbers. But it’s also a massive pain in the … workday. Like, now.Ĭonference calls and online meetings are how we convene in the modern business world. On the personal side, this also helps me manage the near-constant “Mom, can I…?” texts from my kiddos and set reminders to take out the trash on Wednesday nights. I can also set reminders to call a coworker, make a lunch reservation or submit my expense report. The text messaging and email dictation is fantastic, turning rush hour from wasted to productive opportunities for short email responses. The voice input on the Apple Watch might be my favorite interactive feature of all, especially because I’m stuck in Atlanta traffic longer than I care to admit. Here are seven ways smartwatches boost work productivity: 1. But with a plethora of enterprise apps available in the Apple App Store and Google Play Store, IT is seeing an influx of smart, connected devices enter into the workplace-particularly smartwatches.Īs my smartwatch of choice, my Apple Watch productivity game is changing my daily routine for the better. We can monitor our heartbeat, calories, etc. We can track fitness activities throughout the day, such as my husband’s (annoying) obsession with Apple Watch’s reminder to stand up every hour. The beauty of a smartwatch is that it straddles both IoT worlds: consumer and business. For the majority of us, however, IoT primarily means Fitbit or Nike FuelBand-two health and fitness devices that, according to the same study, snagged approximately 52% of the consumer wearables market in 2015.īut since we spend at least a third of our days working, can IoT and wearable devices make us more productive, efficient workers? Absolutely, thanks to connected devices like the Apple Watch, which cornered 52% of the global smartwatch market last year. A Forrester Research study from last September reported that 1-in-5 surveyed adults use a wearable device. ![]() Today, the consumer has a corner on IoT device adoption. Talk abounds about the untapped potential of the Internet of Things ( IoT) at work, but we’re clearly still in early days. Think wearables are only for health and fitness? Think again, fellow workers of the world. ![]() ![]() ![]() In the new update of PUBG Mobile Lite, so many changes have been made to make the game more engaging and play smoothly. The expected PUBG Mobile Lite 0.26.0 update release date is, and the official APK can be downloaded from. What is PUBG Lite 0.26.0 Update Release Date? To make this game compatible with every device, the number of players and map dimension have been reduced, and in this version, only 60 players can play instead of 100 because, for 100 players, you will need a better device and other versions of the game which consume lots of device resources. PUBG MOBILE THE BEST BATTLE ROYALE MOBILE GAME Royale Pass Month 22 New Royale Pass M22 rewards have arrived ENTER More APK download 1.3 GB Download complete. PUBG MOBILE LITE is compatible with more devices and optimized for devices with less RAM without compromising the gameplay experience. Download: PUBG MOBILE LITE APK (Game) - Latest Version: 0.25.0 - Updated: 2023 - Level Infinite - /en-US - Free. Also Download: GBA Emulator Android APK PUBG Mobile Lite 2023 Features Official PUBG Game No need to worry about the authenticity of this game as it is. So rest assured, this is safe to download. It comes directly from the developers of PUBG Mobile. It is a lighter version of the official PUBG Mobile. Here, download PUBG Mobile Lite 0.24.0 APK + OBB via stable channel for Android 13, 12, 11, 10, 9 Pie, 8 Oreo, 7.0 Nougat, 6.0 Marshmallow, or older devices from the following links. PUBG MOBILE LITE 0.25.0 2 variants MaPST Version:0.25.0 Uploaded:Maat 9:21PM PST File size:54.28 MB Downloads:12,214 PUBG MOBILE LITE 0.24.0 2 variants DecemPST Version:0.24.0 Uploaded:Decemat 6:32AM PST File size:54.5 MB Downloads:39,972 PUBG MOBILE LITE 0.23. PUBG Mobile Lite is an online multiplayer battle royale game built with Unreal Engine 4. ![]() Download PUBG MOBILE LITE APK and OBB File.Step 2: They then have to click on the ‘Download APK’ button. Get the full guide on How to Download PUBG Mobile Lite, PUBG Mobile Lite Tips and Tricks, gameplay and much more. ![]() What Is The Difference Between PUBG MOBILE and PUBG MOBILE LITE? Step 1: Players must first visit the official website of PUBG Mobile Lite.Why This Is The Best Battle Royale Mobile Game?.The APK file for the 0.22.0 version is available on the official website, and Pubg Mobile. What is PUBG Lite 0.26.0 Update Release Date? Downloading PUBG Mobile Lites latest version APK download and size. ![]() ![]() ![]() A 5-gallon bucket with a utility lid is a valuable tool for the bridge fisherman. No one needs a hook in the face, and you don’t want your rod ripped out of your hands. ![]() Also, keep watch for small boats that might run through the shallower gaps in bridge pilings. With any bridge fishing technique, avoid fishing directly into a busy boating channel. In areas where grouper frequent rock piles, reefs or channel edges, let the tide drift a diving plug about 50 to 60 yards downstream, then point your rod at the water and reel to pull the plug across the strike zone. Kiting up in the water, the spoon resembles a fleeing baitfish and tempts the toothy fish. Carefully cast the lengthy rig (look behind you first), let the weight drop to the bottom and then reel fast. When mackerel, bluefish, barracuda and other high-speed predators abound, modify the basic Carolina rig by pegging the weight against the bead with a bobber stop, extending the leader to 4 to 5 feet and replacing the hook with a silver spoon. First, consider that angled structures extend much farther than their surface position, so allow appropriate spacing to avoid immediately snagging on barnacles and accumulated sea growth. ![]() That being said, while bridge pilings, abutments and fenders (structures guarding the main channel) attract fish with their current breaks and abundant food sources, fishing near these structures requires measured casts and constant attention. In other words, rather than fishing at a sharp angle beneath the bridge, switch sides (when possible) and fish out and away with greater control. Tide direction will switch throughout the day, so always work with the water for the most natural presentations and the lowest risk of entanglements. test) medium-heavy spinning, baitcasting, trolling, in fresh or salt water for salmon, stelhead, and many salt water species. Check daily tide charts and plan your trip around the optimal flow and consider that the strongest tides occur during the full and new moon cycles, while quarter moons typically bring lesser flow. test) medium spinning, bait casting for large trout, white & black bass, and smaller salt water species. If you must fish strong tides, make an uptide cast under the bridge and let your bait drift naturally away from the structure. Moderate tides are usually the most productive because too much current makes it hard to target specific areas, as your bait moves through the zone too quickly. Generally, fish bite best when the water’s moving, as the tides bring food past bridge pilings, channel edges and any adjacent structure, like construction rubble. ![]() ![]() ![]() Bush, interviewed for a PBS special about his 2000 face-offs with Al Gore, credited Ronald Reagan with elevating the zinger’s importance. But by 1970, as political discourse became less civil and more confrontational, it turned into a catchy synonym for a barbed quip.įormer President George W. ![]() ![]() The Online Etymology Dictionary says that in 1957, it was baseball slang for a pitcher’s fastball that caught unsuspecting hitters off guard. “Zinger” is one of many political words originating in the sports world. Zinger: A supposedly spontaneous clever one-liner that has become a leading measuring stick – to some, the only measuring stick – for success in a debate. That is an awesome responsibility and a revolutionary opportunity. Even when the world is unkind, we can be unmoved in our determination to love, to build, to seek credible hope. Never to excuse or ignore cruelty or crime, but to recognize that how we view the world shapes the world. What is the media’s responsibility?Author and anti-apartheid activist Alan Paton once said of the Monitor, “It gives no shrift to any belief in the irredeemable wickedness of man, nor in the futility of human endeavor.”In addition to reporting acts of kindness, perhaps a next step is to see the world through a lens of kindness. But can this elevation only happen with stories of kindness? Must the rest of the news abandon us to despair?The world is asking us to consider that question deeply. She defined kindness and heroism as “moral beauty,” which “triggers ‘elevation’ – a positive and uplifting feeling” that “acts as an emotional reset button, replacing feelings of cynicism with hope, love and optimism.”The study suggested this happens when one watches a news story about kindness after watching ones about bombings, cruelty, and violence. They support “the belief that the world and people in it are good.” And they provide “relief to the pain we experience when we see others suffering.”It was her fourth point that stuck with me. Since the late 20th century in labor law, quid pro quo is widely used as a name for a type of workplace sexual harassment in which an employer holds an employee’s job hostage in return for sexual favors.A week ago, a British researcher published an article titled “Stories of kindness may counteract the negative effects of looking at bad news.” As you might imagine, I was intrigued.Kathryn Buchanan of the University of Essex shared four main takeaways from her research: Stories of kindness remind us of our shared values. Quid pro quo especially made its way into legal, political, and commerical texts by the 19th century, a useful shorthand for all sorts of reciprocal exchanges. ![]() The phrase was added to a 1535 English translation of Dutch humanist Erasmus, who apparently questioned the questionable quid-pro-quoing of these quacks.īy the late 1500s, quid pro quo spread from medicine into general contexts for a “tit for tat.” A 17th century history on the reign of King Charles, for instance, described Christianity as a quid pro quo in that people must repent for redemption. The expression was notably used in the Middle Ages by apothecaries who were figuring out what substances may be substituted for another ( quid pro quo) in medicines. In Latin, quid pro quo literally means “something for something” or “one thing for another.” ![]() ![]() ![]() In addition, Picasa also provides you with a Side by side editing feature that helps you compare two different photos or the original with the edited version within the same window, while you’re working on it.Īs an added bonus, Picasa makes it easy to preview video files, rapidly upload them to your YouTube account, take quick snapshots and export them to any location of your choice. You also have access to numerous image effects that can be used to further transform your images, as well as a wide variety of tools for fixing and adjusting your photos. Lightroom belongs to one of the best Picasa alternatives due to its Digital Asset Management Library (DAM). Built-in photo editing tools with side by side image editing and comparisonįurthermore, Picasa’s built-in image editor helps you crop your pictures, retouch them, add text and remove red-eyes from the image. The best synchronization capability: ACDSee Photo Studio. Such programs may not work on modern operating. The Google Picasa for Mac software seems to be old or discontinued. Thanks to the Picasa Web Album service, you can also get up to 1 GB of your photos online for simple and straightforward sharing. Picasa was discontinued on March 15, 2016. While using Picasa, you will be able to scan your Mac's hard disk for images and videos and effortlessly and rapidly organize them according to various criteria like creation date, containing folder, user rating and more. ![]() Im lucky enough to have an old version of Picasa installed which can do. Moreover, the Picasa application comes with a user-oriented interface from which you can easily browse and preview your entire video and photo collection, once you’ve added them to your library using the Import toolĪfter you’ve added them to Picasa’s library, your pictures can be rated, geo-tagged, edited and shared with your friends and family via e-mail or your Google+ profile. which places all the photographs in their appropriate geo-locations on Google Earth. ![]() Once you have downloaded the Picasa app, you need to connect your computer to the internet using either a high-speed internet connection or a wireless router, depending upon your location. Picasa is a clean styled and powerful OS X application designed to provide you with a long list of tools and features that can help you organize, edit, adjust and share your photos and videos with ease. It is available free on the Google Play Store and also on the Apple Store. ![]() ![]() ![]() Avoid undercharging, overcharging, or disputes about the number of hours spent on a project. 0:00 / 4:05 How to Reset your Screenshot Counter Anna Navarre 3. Steps: Press the Print Screen key on your keyboard (next to F2 key), and a screenshot of your whole screen will be copied to the clipboard. When you know your team’s capacity, you can accurately plan costs, staffing needs, and more in advance to increase client satisfaction and reduce burnout for your squad.Įnsure accurate data and timekeeping so that when it comes to billing your client, you are 100% sure that your time data is accurate. Reduce the amount of guesswork in resource planning. Record your time-work ratio so you can bill accordingly with Everhour’s time tracking software.Įverhour lets you take all your time data and build easy-to-read time reports that you can share with management or your team to improve productivity. to a procedure that doesnt exist, so the button turns red: Screenshot. Plan your budget and staffing levels in advance. The model well build is a simple ecosystem model not unlike Wolf Sheep Predation. With Everhour’s project management features, you can monitor employee time per project and better plan your estimates for the future. Get to know who was working on which project and when. Our software integrates seamlessly with your tools, so that your employees don’t have to switch between tabs to track when they clocked in and clocked out of the office, or stopped or started working on a particular task. Here’s how our time clock app for business can help:Įverhour’s integrations are some of the most intuitive and user-friendly on the market. ![]() That’s why knowing how much was spent in your business is essential to ensuring those balance sheets stay in the black. Screenshots are taken randomly within 10 minutes interval. Timers page shows what your team is doing right now and their screenshots.Įach screenshot has exact time when it was taken and a task title for which a user was working at the moment of screenshot taking. Web app or browser extension can't capture screenshots. Screenshots are captured only if you have the screenshot recording app installed and running. Step 3: Install and run our screenshot recording app Just like you can select specific team members who should use the screenshots app. Screenshots app can be turned on or off at any time. How to count cells with a hemocytometer What rules and strategies should I use for manual cell counting How do I calculate dilution factor, cell count, and. Next, invite your team.Ĭapturing screenshots is an essential yet not used by every team functionality. In just a few steps, you’ll have your time tracking account up and running. Step 1: Create your FREE Everhour accountĬlick here and sign up. 0:00 / 4:05 How to Reset your Screenshot Counter Anna Navarre 3.64K subscribers Subscribe 343 views 1 year ago It is super easy to reset your screenshot counter index, but since it isnt. That’s why we designed our screenshot recording module to be extremely lightweight. It was crafted with Swift, optimized for M1, and is distributed for free. We believe that time tracking should be easy. Download Shottr for Mac, v1.6, 1.3mb v1.6.2 is out, check release notes Shottr is a small, fast, human-sized screenshot app built for those who care about pixels. How to use Everhour's time tracking software with screenshots? ![]() ![]() ![]() The application of CRISPR-Cas9 in tomato genome engineering obtained targeted mutations at four different loci. Therefore, new tomato cultivars capable of biotic and abiotic stress tolerance are important subjects of breeding using GE technology. Recently, unforeseen climate changes have caused many biotic and abiotic stresses that negatively affect multiple aspects of tomato production, such as yield and quality, thereby threatening agriculture sustainability. It has been successful in modifying tomato genomes and precision breeding to create new alleles, not including linkage drag ( Zsögön et al., 2017 Li et al., 2018b Zsögön et al., 2018 Vu et al., 2020a). CRISPR-Cas-based GE has been effectively applied for plant breeding at high precision levels ( Chen et al., 2019). Genome editing (GE) technology using CRISPR-Cas nucleases has recently emerged as a revolutionary plant breeding technology and shows promise to the agriculture industry ( Belhaj et al., 2013 Chen et al., 2019 Vu et al., 2022). CRISPR-Cas9-based precise domain editing of the SlHyPRP1 gene generated multi-stress-tolerant alleles that could be used as genetic materials for tomato breeding. However, the edited alleles enhanced susceptibility against Fusarium oxysporum f. tomato ( Pto) DC3000, the growth of the bacterium was significantly reduced by 2.0- to 2.5-fold compared to that in WT plants. When the edited lines were challenged with pathogenic bacteria of Pseudomonas syringae pv. Moreover, the PR1v1 line continuously grew after 5 days of water cutoff. Similarly, plants carrying either the domains of the PRD removal variant (PR1v1) or 8CM removal variants (PR2v2 and PR2v3) showed better germination under osmosis stress (up to 200 mM mannitol) compared to the WT control. Our data reveal that the 8CM removal variants of HK and the KO alleles of both HK and 15T01 cultivars exhibited moderate heat stress tolerance. In this study, we characterized the edited lines under several abiotic and biotic stresses to examine the possibility of multiple stress tolerance. We subsequently demonstrated that eliminating the PRD domain of HyPRP1 in tomatoes conferred the highest level of salinity tolerance. In our previous report, a tomato gene encoding hybrid proline-rich protein 1 (HyPRP1), a negative regulator of salt stress responses, has been edited using a CRISPR-Cas9 multiplexing approach that resulted in precise eliminations of its functional domains, proline-rich domain (PRD) and eight cysteine-motif (8CM). Recently, CRISPR-Cas9-based genome editing has been widely used for plant breeding. 6Nulla Bio R&D Center, Nulla Bio Inc., Jinju, Republic of Korea.5National Key Laboratory for Plant Cell Biotechnology, Agricultural Genetics Institute, Hanoi, Vietnam.4Division of Life Science, Gyeongsang National University, Jinju, Republic of Korea.3Division of Horticultural Science, Gyeongsang National University, Jinju, Republic of Korea. ![]() 2Crop Science and Rural Development Division, College of Agriculture, Bac Lieu University, Bac Lieu, Vietnam.1Division of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Republic of Korea.The pasted sequence should be FASTA format.Mil Thi Tran 1,2,3†, Geon Hui Son 1†, Young Jong Song 1†, Ngan Thi Nguyen 1, Seonyeong Park 1, Thanh Vu Thach 1, Jihae Kim 1, Yeon Woo Sung 1, Swati Das 1, Dibyajyoti Pramanik 1, Jinsu Lee 1, Ki-Ho Son 3, Sang Hee Kim 1,4*‡, Tien Van Vu 1,5*‡ and Jae-Yean Kim 1,4,6*‡ Please choose the targets to test and/or paste a custom sequence into the text area below. Select to check for secondary off-targets Tolerated edit distance to the target sequenceĬheck off-targets in non-gene regions should be ignored Retrieve and save a recombination donor matrixĪssess restriction sites of the whole sequence Įxclude designs with more than X off-targets Number of coding exons downstream the start codon for K.O.īp Minimal spacer length for paired designsīp Maximum spacer length for paired designs Select if CRISPR designs should be used for tagging or knockout experimentsīp Minimum guide RNA length after PAM īp Maximum guide RNA length after PAM īp Tagging window downstream of the codon (only NGG PAM, only G as 5' base, off-target tolerates many mismatches and ignores non-seed region, introns, purpose is knockout (only first 3 coding exons are allowed) and UTRs are excluded) (any PAM (NAG/NGG.), any 5' base (A,C,G,T.), off-targets tolerate mismatches, introns/CPG islands are excluded) (any PAM (NAG/NGG.), any 5' base (A,C,G,T.), off-targets need full length perfect match, introns are allowed) ![]() |